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Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples
Oborny, Nathan John Melo Costa, Elton E. Suntornsuk, Leena Abreu, Fabiane Lunte, Susan M.
Oborny, Nathan John
Melo Costa, Elton E.
Suntornsuk, Leena
Abreu, Fabiane
Lunte, Susan M.
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Abstract
A portable fluorescence detection system for use with microchip electrophoresis was developed and compared to a benchtop system. Using this system, six neuroactive amines commonly found in brain dialysate (arginine, citrulline, taurine, histamine, glutamate, and aspartate) were derivatized offline with naphthalene-2,3-dicarboxaldehyde/cyanide, separated electrophoretically, and detected by fluorescence. The limits of detection for the analytes of interest were 50 – 250 nM for the benchtop system and 250 nM – 1.3 μM for the portable system, both of which were adequate for most analyte detection in brain microdialysis samples. The portable system was then demonstrated for the detection of the same six amines in a rat brain microdialysis sample.
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Date
2016-01-10
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Nihon Bunseki Kagakkai
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Keywords
Microchip electrophoresis, Amino acid neurotransmitter, Microdialysis, Fluorescence detection, Naphthalene-2, 3-dicarboxaldehyde, Neuroactive amine
Citation
Oborny, N. J., Costa, E. E., Suntornsuk, L., Abreu, F. C., & Lunte, S. M. (2016). Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples. Analytical Sciences, 32(1), 35-40. doi:10.2116/analsci.32.35