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Hybrid near-field scanning optical microscopy tips for live cell measurements
Kapkiai, Luka K. Moore-Nichols, David Carnell, Jonathan Krogmeier, Jeffrey R. Dunn, Robert C.
Kapkiai, Luka K.
Moore-Nichols, David
Carnell, Jonathan
Krogmeier, Jeffrey R.
Dunn, Robert C.
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Abstract
We report a near-field scanning optical microscopy (NSOM) probe that enables high-resolution imaging of living cells under physiological buffered conditions. The hybrid design combines a conventional fiber optic near-field probe with a standard atomic force microscopy cantilever. Imaging of fluorescent latex spheres suspended in an acetate matrix demonstrates the subdiffraction limited fluorescence and topography capabilities of the tips. The reduced spring constant of the hybrid tip is also shown to be amenable to measurements on living cells. NSOM fluorescence and topography measurements on living human arterial smooth muscle cells under buffered conditions are demonstrated.
Description
This is the published version, also available here: http://dx.doi.org/10.1063/1.1737464.
Date
2004-03-08
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American Institute of Physics
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Keywords
Fluorescence, Near-field scanning optical microscopy, Topography, Atomic force microscopy, Fiber optics
Citation
Kapkiai, Luka K., Moore-Nichols, David., Carnell, Jonathan., Krogmeier, Jeffrey R., Dunn, Robert C. "Hybrid near-field scanning optical microscopy tips for live cell measurements." Appl. Phys. Lett. 84, 3750 (2004); http://dx.doi.org/10.1063/1.1737464.