Loading...
Optimization of flavin oxidase enzymes for human health monitoring
Deay, Dwight O'Dell
Deay, Dwight O'Dell
Citations
Altmetric:
Abstract
Biosensors are an analytic modality able to quantify, in real time, the abundance of specific analytes in complex biological mixtures such as blood, the brain, and in a variety of organs. However, the use of biosensors has been limited to analytes for which suitable oxidase enzyme exists. Flavin dependent peroxide producing oxidase enzymes serve as the highly specific biorecognition elements in the fabrication of amperometric biosensors. While many enzymes have been evaluated for the purposes of in vivo sensing, most of these studies have failed because these enzymes are inherently unsuitable for in vivo biosensing applications because of low stability, low substrate affinity, and/or slow rates of turnover. The common thread that ties together these diverse studies performed as part of my dissertation work is the focus on improving enzymes for in vivo biosensing and human health monitoring applications. My goal has been to improve the toolbox available to the electrochemists and developers of biosensors by engineering oxidase enzymes with improved performance in vivo and in vitro biosensing applications. Specifically, my dissertation work focuses primarily on two critical aspects of biosensor design: (1) the structure-informed rational improvement of three FDPGOs as potential nicotine oxidases and (2) the successful use of a gold binding peptide fusion sequence for the orientation-controlled immobilization of a putrescine oxidase on gold surfaces.
Description
Date
2021-05-31
Journal Title
Journal ISSN
Volume Title
Publisher
University of Kansas
Collections
Research Projects
Organizational Units
Journal Issue
Keywords
Biochemistry, Biophysics, Biosensing, Biosensor, Flavin Oxidase, Metal binding Peptide, Nicotine Oxidase, Protein engineering