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Glycoprotein profiling by electrospray mass spectrometry
Jiang, Hui Desaire, Heather Butnev, Vladimir Y. Bousfield, George R.
Jiang, Hui
Desaire, Heather
Butnev, Vladimir Y.
Bousfield, George R.
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Abstract
This work compares several different methods of site-specific analysis of glycoproteins using electrospray mass spectrometry. The glycoprotein, oLHalpha (ovine luteinizing hormone, a-subunit) was chosen as an appropriate example protein for these studies because of its biological relevance and extreme microheterogeneity. More than 20 unique glycoforms were detected for this glycoprotein at the Asn(56) site of oLHalpha. The carbohydrates present at this site affect receptor binding affinity, so understanding the great variety in the composition of these carbohydrates is important in studying ligand binding interactions. MS data was acquired on a quadrupole ion trap, a triple quadrupole, and a quadrupole time of flight mass spectrometer, and carbohydrate composition at the Asn(56) site of oLHalpha was determined using these instruments. Additionally, neutral loss and precursor ion scanning modes were also used to identify the glycoforms present, and these techniques were compared to the standard MS data. Of the three instruments compared in the study, the qTOF mass spectrometer achieved the lowest sample consumption, but all three instruments were useful in profiling the glycopeptide composition. (C) 2004 American Society for Mass Spectrometry.
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Date
2004-05
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ELSEVIER SCIENCE INC
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Keywords
Analytical Chemistry, Physical Chemistry, Spectroscopy
Citation
Jiang, H; Desaire, H; Butnev, VY; Bousfield, GR. Glycoprotein profiling by electrospray mass spectrometry. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY. May 2004. 15(5):750-758.