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Comparison of molecular typing methods for Candida albicans.
Magee, P. T. ; Bowdin, Linda ; Staudinger, Jeffrey Leonard
Magee, P. T.
Bowdin, Linda
Staudinger, Jeffrey Leonard
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Abstract
Four molecular approaches to determining the types of Candida albicans strains were compared. The strains used were those whose repeated DNA (ribosomal and mitochondrial) EcoRI restriction fragment length polymorphisms (RFLP) were determined by Stevens et al. (D. A. Stevens, F. C. Odds, and S. Scherer, Rev. Infect. Dis. 12:258-266, 1990). Scherer and Stevens (S. Scherer and D. A. Stevens, Proc. Natl. Acad. Sci. USA 85:1452-1456, 1988) used the same strains to examine the Southern blots of genomic EcoRI digests probed with the repeated sequence 27A. The results of these investigators were compared with determinations of RFLPs generated from repeated DNA by the enzyme HinfI and examination of the karyotypes of strains under two sets of conditions, one for the smaller chromosomes and one for the larger ones. Analysis of RFLPs of repeated DNA is most convenient but shows the lowest degree of resolution. Use of the repeated sequence and use of karyotype have very high resolution, but the former method is more convenient than the latter. HinfI digestion is more sensitive than EcoRI digestion but equally convenient. By using all four methods, separate types were identified for 18 of the 20 strains examined.
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This is the published version. Copyright 1992 American Society for Microbiology.
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1992-10
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American Society for Microbiology
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Magee, P. T., L. Bowdin, and Jeffrey Leonard Staudinger. "Comparison of Molecular Typing Methods for Candida Albicans." Journal of Clinical Microbiology 30.10 (1992): 2674-679. Web. 27 May 2015.