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Viscoelastic Properties of ECM-Rich Embryonic Microenvironments
Akos, Zsuzsa Isai, Dona Greta Rajasingh, Sheeja Kosa, Edina Ghazvini, Saba Dhar, Prajnaparamita Czirok, Andras
Akos, Zsuzsa
Isai, Dona Greta
Rajasingh, Sheeja
Kosa, Edina
Ghazvini, Saba
Dhar, Prajnaparamita
Czirok, Andras
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Abstract
The material properties of tissues and their mechanical state is an important factor in development, disease, regenerative medicine and tissue engineering. Here we describe a microrheological measurement technique utilizing aggregates of microinjected ferromagnetic nickel particles to probe the viscoelastic properties of embryonic tissues. Quail embryos were cultured in a plastic incubator chamber located at the center of two pairs of crossed electromagnets. We found a pronounced viscoelastic behavior within the ECM-rich region separating the mesoderm and endoderm in Hamburger Hamilton stage 10 quail embryos, consistent with a Zener (standard generalized solid) model. The viscoelastic response is about 45% of the total response, with a characteristic relaxation time of 1.3 s.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
Date
2020-08-31
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Frontiers Media
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Keywords
ECM, Quail embryo, Microrheology, Elasticity, Young’s modulus, Zener solid, Magnetic, Nanorods
Citation
Akos, Z., Isai, D. G., Rajasingh, S., Kosa, E., Ghazvini, S., Dhar, P., & Czirok, A. (2020). Viscoelastic Properties of ECM-Rich Embryonic Microenvironments. Frontiers in cell and developmental biology, 8, 674. https://doi.org/10.3389/fcell.2020.00674