Loading...
Cytoplasmic dynein’s mitotic spindle pole localization requires a functional anaphase-promoting complex, γ-tubulin, and NUDF/LIS1 in Aspergillus nidulans
Li, Shihe Oakley, C. Elizabeth Chen, Guifang Han, Xiaoyan Oakley, Berl R. Xiang, Xin
Li, Shihe
Oakley, C. Elizabeth
Chen, Guifang
Han, Xiaoyan
Oakley, Berl R.
Xiang, Xin
Citations
Altmetric:
Abstract
In Aspergillus nidulans, cytoplasmic dynein and NUDF/LIS1 are found at the spindle poles during mitosis, but they seem to be targeted to this location via different mechanisms. The spindle pole localization of cytoplasmic dynein requires the function of the anaphase-promoting complex (APC), whereas that of NUDF does not. Moreover, although NUDF's localization to the spindle poles does not require a fully functional dynein motor, the function of NUDF is important for cytoplasmic dynein's targeting to the spindle poles. Interestingly, a γ-tubulin mutation, mipAR63, nearly eliminates the localization of cytoplasmic dynein to the spindle poles, but it has no apparent effect on NUDF's spindle pole localization. Live cell analysis of the mipAR63 mutant revealed a defect in chromosome separation accompanied by unscheduled spindle elongation before the completion of anaphase A, suggesting that γ-tubulin may recruit regulatory proteins to the spindle poles for mitotic progression. In A. nidulans, dynein is not apparently required for mitotic progression. In the presence of a low amount of benomyl, a microtubule-depolymerizing agent, however, a dynein mutant diploid strain exhibits a more pronounced chromosome loss phenotype than the control, indicating that cytoplasmic dynein plays a role in chromosome segregation.
Description
This is the publisher's version, also available electronically from "http://www.molbiolcell.org".
Date
2005-08-01
Journal Title
Journal ISSN
Volume Title
Publisher
American Society for Cell Biology
Collections
Research Projects
Organizational Units
Journal Issue
Keywords
Citation
Li, S., Oakley, C., Chen, G., Han, X., Oakley, B., & Xiang, X. (2005). Cytoplasmic dynein’s mitotic spindle pole localization requires a functional anaphase-promoting complex, γ-tubulin, and NUDF/LIS1 in Aspergillus nidulans. Moleculary Biology of the Cell, 16(8), 3591-3605. http://www.dx.doi.org/10.1091/mbc.E04-12-1071