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A Methodology for Simultaneous Fluorogenic Derivatization and Boronate Affinity Enrichment of 3-Nitrotyrosine Containing Peptides
Dremina, Elena S. Li, Xiaobao Galeva, Nadezhda A. Sharov, Victor S. Stobaugh, John F. Schoneich, Christian
Dremina, Elena S.
Li, Xiaobao
Galeva, Nadezhda A.
Sharov, Victor S.
Stobaugh, John F.
Schoneich, Christian
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Abstract
We synthesized and characterized a new tagging reagent, (3R,4S)-1-(4-(aminomethyl)phenylsulfonyl)pyrrolidine-3,4-diol (APPD), for the selective fluorogenic derivatization of 3-nitrotyrosine (3-NT) residues in peptides (after reduction to 3-aminotyrosine) and affinity enrichment. The synthetic 3-NT-containing peptide, FSAY(3-NO2)LER, was employed as a model for method validation. Further, this derivatization protocol was successfully tested for analysis of 3-NT-containing proteins exposed to peroxynitrite in the total protein lysate of cultured C2C12 cells. The quantitation of 3-NT content in samples was achieved through either fluorescence spectrometry or boronate affinity chromatography with detection by specific fluorescence (excitation and emission wavelengths of 360 and 510 nm, respectively); the respective limits of detection were 95 and 68 nM (19 and 13 pmol total amount) of 3-NT. Importantly, the derivatized peptides show a strong retention on a synthetic boronate affinity column, containing sulfonamide-phenylboronic acid, under mild chromatographic conditions, affording a route to separate the derivatized peptides from large amounts (milligrams) of non-derivatized peptides, and to enrich them for fluorescent detection and MS identification. Tandem MS analysis identified chemical structures of peptide 3-NT fluorescent derivatives and revealed that the fluorescent derivatives undergo efficient backbone fragmentations, permitting sequence-specific identification of protein nitration at low concentrations of 3-NT in complex protein mixtures.
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Date
2012-11-15
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Elsevier Masson
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Keywords
3-Nitrotyrosine, (3R, 4S)-1-(4-(aminomethyl)phenylsulfonyl) pyrrolidine-3,4-diol (APPD), Fluorogenic derivatization, Fluorescence, Boronate affinity chromatography, Mass spectrometry, Proteomics
Citation
Dremina, Elena S., Xiaobao Li, Nadezhda A. Galeva, Victor S. Sharov, John F. Stobaugh, and Christian Schöneich. "A Methodology for Simultaneous Fluorogenic Derivatization and Boronate Affinity Enrichment of 3-nitrotyrosine-containing Peptides." Analytical Biochemistry 418.2 (2011): 184-96.