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Quantification and characterization of biological activities of glansreginin A in black walnuts (Juglans nigra)
Ho, Khanh-Van Hsieh, Hsien-Yeh Roy, Anuradha Foote, Sarah McDonald, Peter Coggeshall, Mark V. Ito, Hideyuki Lei, Zhentian Sumner, Lloyd W. Stewart, George C.
Ho, Khanh-Van
Hsieh, Hsien-Yeh
Roy, Anuradha
Foote, Sarah
McDonald, Peter
Coggeshall, Mark V.
Ito, Hideyuki
Lei, Zhentian
Sumner, Lloyd W.
Stewart, George C.
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Abstract
Glansreginin A has been reported to be an indicator of the quality of walnuts (Juglans spp.). However, bioactive properties of glansreginin A have not been adequately explored. In the present study, we quantified concentrations of glansreginin A in black walnuts (Juglans nigra) using high performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) and performed an array of in vitro bioassays to characterize biological activities (e.g., antibacterial, antioxidant, anticancer capacities) of this compound. Results from HPLC–MS/MS analysis indicated that glansreginin A was presented in all 12 black cultivars examined and its contents were variable among black walnut cultivars, ranged from 6.8 mg/kg (Jackson) to 47.0 mg/kg (Hay). Glansreginin A possessed moderate antibacterial activities against Gram-positive pathogens (Staphylococcus aureus and Bacillus anthracis). This compound exhibited no antioxidant activities, did not induce the activity of antioxidant response element signaling pathways, and exerted no antiproliferative effects on tumorigenic alveolar epithelial cells and non-tumorigenic lung fibroblast cells.
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Date
2023-11-01
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Publisher
Nature Research
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Keywords
Mass spectrometry, Assay systems
Citation
Ho KV, Hsieh HY, Roy A, Foote S, McDonald P, Coggeshall MV, Ito H, Lei Z, Sumner LW, Stewart GC, Lin CH. Quantification and characterization of biological activities of glansreginin A in black walnuts (Juglans nigra). Sci Rep. 2023 Nov 1;13(1):18860. doi: 10.1038/s41598-023-46134-8. PMID: 37914763; PMCID: PMC10620390