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A COMPARISON OF 2D IMAGE ANALYSIS AND DESIGN-BASED STEREOLOGY FOR EVALUATING MORPHOLOGICAL AND ANATOMICAL CHANGES IN THE DOPAMINERGIC SYSTEM OF THE RODENT MIDBRAIN
Park, Ji-Hyuk
Park, Ji-Hyuk
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Abstract
Background. 2D analyses produce systematic errors in quantifying anatomical and morphological features in the brain. Design-based stereology overcomes this limitation by applying probability theory, yet many neuroscience investigators still use 2D analyses. The purpose of this study is to compare 2D analysis with design-based stereology in quantifying differences of morphological and anatomical features between groups. Methods. Brain tissue samples of three different rodent models were analyzed; chronic MPTP/probenecid PD (MPD) mouse model, alcohol preferring (AP) rat model, and the enriched environment (EE) rat model. 2D analyses and design-based stereology were used to quantify neuronal number, neuronal volume and regional volume. Student's t-test (two-tailed) was used to compare quantitative data. Results. 2D analyses generated significantly different estimation form design-based stereology in neuronal number and did not find relatively small differences of neuronal number. 2D analysis generated comparable value to design-based stereology in normalized data but not in actual value. 2D estimated accurately regional volume. Discussion. 2D analyses may be used for rough screening to find a difference of neuronal number and volume but should not for the estimation of actual value. Design-based stereology should be used to estimate neuronal number and volume. Both 2D analyses and design based stereology can be used for the estimation of regional volume.
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Date
2010-06-13
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University of Kansas
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Keywords
Nanoscience, Biology, Morphology