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Publication Kinship practices at the early bronze age site of Leubingen in Central Germany(Nature Research, 2024-02-16) Haak, WolfgangWith the beginning of the Early Bronze Age in Central Europe ~ 2200 BC, a regional and supra-regional hierarchical social organization emerged with few individuals in positions of power (chiefs), set apart by rich graves with extensive burial constructions. However, the social organization and stratification within the majority of people, who represent the non-elite, remain unclear. Here, we present genome-wide data of 46 individuals from the Early Bronze Age burial ground of Leubingen in today's Germany, integrating archaeological, genetic and strontium isotope data to gain new insights into Early Bronze Age societies. We were able to reconstruct five pedigrees which constitute the members of close biological kinship groups (parents and their offspring), and also identify individuals who are not related to individuals buried at the site. Based on combined lines of evidence, we observe that the kinship structure of the burial community was predominantly patrilineal/virilocal involving female exogamy. Further, we detect a difference in the amount of grave goods among the individuals buried at Leubingen based on genetic sex, age at death and locality but see no difference in the types of grave goods.Publication Review: Computational analysis of human skeletal remains in ancient DNA and forensic genetics(Elsevier, 2023-10-04) Zavala, Elena IDegraded DNA is used to answer questions in the fields of ancient DNA (aDNA) and forensic genetics. While aDNA studies typically center around human evolution and past history, and forensic genetics is often more concerned with identifying a specific individual, scientists in both fields face similar challenges. The overlap in source material has prompted periodic discussions and studies on the advantages of collaboration between fields toward mutually beneficial methodological advancements. However, most have been centered around wet laboratory methods (sampling, DNA extraction, library preparation, etc.). In this review, we focus on the computational side of the analytical workflow. We discuss limitations and considerations to consider when working with degraded DNA. We hope this review provides a framework to researchers new to computational workflows for how to think about analyzing highly degraded DNA and prompts an increase of collaboration between the forensic genetics and aDNA fields.Publication Beringia and the peopling of the Western Hemisphere(The Royal Society, 2023-01-11) Hoffecker, John F.; Elias, Scott A.; Scott, G. Richard; O’Rourke, Dennis H.; Hlusko, Leslea J.; Potapova, Olga; Pitulko, Vladimir; Pavlova, Elena; Bourgeon, Lauriane; Vachula, Richard S.Did Beringian environments represent an ecological barrier to humans until less than 15 000 years ago or was access to the Americas controlled by the spatial–temporal distribution of North American ice sheets? Beringian environments varied with respect to climate and biota, especially in the two major areas of exposed continental shelf. The East Siberian Arctic Shelf (‘Great Arctic Plain’ (GAP)) supported a dry steppe-tundra biome inhabited by a diverse large-mammal community, while the southern Bering-Chukchi Platform (‘Bering Land Bridge’ (BLB)) supported mesic tundra and probably a lower large-mammal biomass. A human population with west Eurasian roots occupied the GAP before the Last Glacial Maximum (LGM) and may have accessed mid-latitude North America via an interior ice-free corridor. Re-opening of the corridor less than 14 000 years ago indicates that the primary ancestors of living First Peoples, who already had spread widely in the Americas at this time, probably dispersed from the NW Pacific coast. A genetic ‘arctic signal’ in non-arctic First Peoples suggests that their parent population inhabited the GAP during the LGM, before their split from the former. We infer a shift from GAP terrestrial to a subarctic maritime economy on the southern BLB coast before dispersal in the Americas from the NW Pacific coast.Publication Palaeo-Eskimo genetic ancestry and the peopling of Chukotka and North America(Nature Research, 2019-06-05) Flegontov, Pavel; Altınışık, N. Ezgi; Changmai, Piya; Rohland, Nadin; Mallick, Swapan; Adamski, Nicole; Bolnick, Deborah A.; Broomandkhoshbacht, Nasreen; Candilio, Francesca; Culleton, Brendan J.; Flegontova, Olga; Friesen, T. Max; Jeong, Choongwon; Harper, Thomas K.; Keating, Denise; Kennett, Douglas J.; Kim, Alexander M.; Lamnidis, Thiseas C.; Lawson, Ann Marie; Olalde, Iñigo; Oppenheimer, Jonas; Potter, Ben A.; Raff, Jennifer; Sattler, Robert A.; Skoglund, Pontus; Stewardson, Kristin; Vajda, Edward J.; Vasilyev, Sergey; Veselovskaya, Elizaveta; Hayes, M. Geoffrey; O’Rourke, Dennis H.; Krause, Johannes; Pinhasi, Ron; Reich, David; Schiffels, StephanMuch of the American Arctic was first settled 5,000 years ago, by groups of people known as Palaeo-Eskimos. They were subsequently joined and largely displaced around 1,000 years ago by ancestors of the present-day Inuit and Yup’ik1,2,3. The genetic relationship between Palaeo-Eskimos and Native American, Inuit, Yup’ik and Aleut populations remains uncertain4,5,6. Here we present genomic data for 48 ancient individuals from Chukotka, East Siberia, the Aleutian Islands, Alaska, and the Canadian Arctic. We co-analyse these data with data from present-day Alaskan Iñupiat and West Siberian populations and published genomes. Using methods based on rare-allele and haplotype sharing, as well as established techniques4,7,8,9, we show that Palaeo-Eskimo-related ancestry is ubiquitous among people who speak Na-Dene and Eskimo–Aleut languages. We develop a comprehensive model for the Holocene peopling events of Chukotka and North America, and show that Na-Dene-speaking peoples, people of the Aleutian Islands, and Yup’ik and Inuit across the Arctic region all share ancestry from a single Palaeo-Eskimo-related Siberian source.Publication Laman KiKongo Collection Guide (and Supplementary Materials)(2021-04-21) Janzen, John M.; MacGaffey, WyattThis collection is an archival treasure of about 10,000 handwritten pages that records the life of people in the Lower Congo early in the 20th century. Congolese teachers wrote the notebooks in response to questions prepared by Karl Laman, missionary ethnographer linguist with the Swedish Covenant Church from 1898 until 1917. The notebooks cover the history of the region where the teachers worked (see map) and its peoples; their livelihoods, food preparation and domestic housing; social organization, political structures, religious beliefs and prominent rituals; language arts, music, dance, visual and material culture. Some of the authors depart from the questions to offer creative independent narratives on topics of their interest. Throughout the decade in which he developed his lexicon for the dictionary, Laman corresponded with scholars about his work and extended his questionnaire with the teachers and discussed with them the nature of their findings. The collection represents a veritable encyclopedia on Kongo culture in the Kikongo language by Kongo authors.Publication Tibetan as a dominant Sprachbund language: its interactions with neighboring languages(New York: Trace Foundation., 2013) Dwyer, Arienne M.The Amdo plateau is well-known as a language convergence area (Dwyer 1995, Slater 2003, Janhunen 2004). But what kind of influence has Tibetan had on the languages of that region? What cultural contacts have facilitated or hindered convergence? This paper presents an over- view of contacts between varieties of Tibetan and the languages of western China and Chinese Turkestan, and then focuses on the Amdo Sprachbund as a site of intensive contact with Mongo- lic, Turkic and Sinitic. Typically as a superstrate language, Tibetan has contributed to creoliza- tion processes in the many unrelated local languages, and even in northwestern Mandarin. In ad- dition to phonological and lexical convergence, many of these languages —Monguor, Baonan, Santa, Wutun, Kangjia and Salar— show evidence of syntactic-semantic convergence. Most of these non-dominant languages now have a modified Tibetan evidential system. Cultural convergence is also evident in kinship relations, lifecycle practices, and oral folklore. Most of the above non-Tibetan groups, for example, tell varieties of the Gesar epic. The paper explores the extent to which cultural convergence facilitated linguistic convergence, and illustrates the degree to which Tibetan linguistic and cultural practices are embedded in these non-Tibetan languages and cultures.Publication Dataset on the epidemiology and genetic diversification of dengue virus (DENV) serotypes and genotypes in Mexico(Elsevier, 2020-07-29) Domínguez-de-la-Cruz, Eduardo; de Lourdes Muñoz, María; Hernández-García, Ericel; Pérez-Ramírez, Gerardo; David, Randy E.; Navarrete-Espinosa, Joel; Díaz-Badillo, Álvaro; Moreno-Galeana, Miguel; Brito-Carreón, Cesar ArmandoDengue virus (DENV) evolution has had a significant impact on disease pathogenesis, virulence, and epidemiology in Mexico. Novel genotypic variation in DENV serotypes and genotypes may influence the magnitude and severity of dengue epidemics, as evidenced by 2009 data from Veracruz State. The data presented herein is related to the publication entitled “Epidemiological Implications of the Genetic Diversification of Dengue Virus (DENV) Serotypes and Genotypes in Mexico” [1]. Raw data and trees provide epidemiological data on DENV prevalence and a comprehensive phylogeny of both representative sequences collected from an NCBI repository, and 28 additional isolates from acute-phase plasma samples diagnosed with dengue fever or severe dengue (Raw sequencing data is hosted in the public repository Mendeley Data (http://dx.doi.org/10.17632/bf2kdhhf6x.2). Phylogenetic trees for each DENV serotype (DENV-1, -2, -3 and -4) were constructed using these sequences by a maximum likelihood methodology as well as a Bayesian Markov chain Monte Carlo (MCMC) integration approach. Phylogenetic trees exhibited: (1) DENV-1, genotype V, (2) the DENV-2 Asian/American and Asian II genotypes, (3) DENV-3, genotype III, and (4) DENV-4, genotype I. This data can be beneficial for future analyses on DENV serotype and genotype structure and the introduction of novel DENV genotype sequences in the Americas, for the further elucidation of dengue etiology.Publication Surface analysis of an eagle talon from Krapina(Nature Research, 2020-04-14) Radovčić, Davorka; Birarda, Giovanni; Sršen, Ankica Oros; Vaccari, Lisa; Radovčić, Jakov; Frayer, David W.The Krapina white-tailed eagle talons represent a kind of jewelry worn by Krapina Neandertals some 130,000 years ago. New inspection of one Krapina talon (386.1) revealed a fiber, sealed by a thin silicate coating, adhering to the surface within a wide cut mark, as well as concentrated traces of occasional spots of red and yellow pigment and some black stains. We analyzed the fiber and small portions of pigmented areas by non-invasive, infrared synchrotron beam. Different areas were targeted, revealing the protein nature of the fiber, identified as of animal origin. Targeted areas revealed intra- and inter-strand aggregation indicating the fiber to be collagen losing its original triple α-helix conformation, further confirming the diagenetic decay of the original collagen structure and the antiquity of the fiber. It is possible that the fiber is a remnant of the leather or sinew string binding the talons together. Spectroscopic analysis of the pigments in two isolated areas confirmed two types of ochre and that the dark spots are charcoal remnants. Applying novel non-invasive technologies provides new possibilities to further test the hypothesis of using prehistoric objects for symbolic purposes.Publication A dictionary of Eastern Bonan(2020-06-12) Li, Charles N.; Dwyer, Arienne M.Publication XQuery databases for linguistic resources in the IAIA and UyLVs projects(2013-07) Sperberg-McQueen, C.M.; Dwyer, Arienne M.Increasing numbers of linguists have multimodal data of relatively rare languages, richly annotated for grammatical information. Querying these data, in the service of linguistic discovery, should be powerful and flexible. This paper explores both the promise of linguistic discovery, as well as the problems of open-ended querying and sibling searches: How to balance maximum accessiblity while attending to security and allowing open-ended queries? How to optimize searches for noncontiguous morphemes of a sentence?Publication A population-specific reference panel empowers genetic studies of Anabaptist populations(Nature Research, 2017-07-20) Hou, Liping; Kember, Rachel L.; Roach, Jared C.; O'Connell, Jeffrey R.; Craig, David W.; Bucan, Maja; Scott, William K.; Pericak-Vance, Margaret; Haines, Jonathan L.; Crawford, Michael H.; Shuldiner, Alan R.; McMahon, Francis J.Genotype imputation is a powerful strategy for achieving the large sample sizes required for identification of variants underlying complex phenotypes, but imputation of rare variants remains problematic. Genetically isolated populations offer one solution, however population-specific reference panels are needed to assure optimal imputation accuracy and allele frequency estimation. Here we report the Anabaptist Genome Reference Panel (AGRP), the first whole-genome catalogue of variants and phased haplotypes in people of Amish and Mennonite ancestry. Based on high-depth whole-genome sequence (WGS) from 265 individuals, the AGRP contains >12 M high-confidence single nucleotide variants and short indels, of which ~12.5% are novel. These Anabaptist-specific variants were more deleterious than variants with comparable frequencies observed in the 1000 Genomes panel. About 43,000 variants showed enriched allele frequencies in AGRP, consistent with drift. When combined with the 1000 Genomes Project reference panel, the AGRP substantially improved imputation, especially for rarer variants. The AGRP is freely available to researchers through an imputation server.Publication Environmental selection during the last ice age on the mother-to-infant transmission of vitamin D and fatty acids through breast milk(2018-03-23) O'Rourke, Dennis H.; Hlusko, Leslea J.; Carlson, Joshua P.; Chaplin, George; Elias, Scott A.; Hoffecker, John F.; Huffman, Michaela; Jablonski, Nina G.; Monson, Tesla A.; Pilloud, Marin A.; Scott, G. RichardBecause of the ubiquitous adaptability of our material culture, some human populations have occupied extreme environments that intensified selection on existing genomic variation. By 32,000 years ago, people were living in Arctic Beringia, and during the Last Glacial Maximum (LGM; 28,000–18,000 y ago), they likely persisted in the Beringian refugium. Such high latitudes provide only very low levels of UV radiation, and can thereby lead to dangerously low levels of biosynthesized vitamin D. The physiological effects of vitamin D deficiency range from reduced dietary absorption of calcium to a compromised immune system and modified adipose tissue function. The ectodysplasin A receptor (EDAR) gene has a range of pleiotropic effects, including sweat gland density, incisor shoveling, and mammary gland ductal branching. The frequency of the human-specific EDAR V370A allele appears to be uniquely elevated in North and East Asian and New World populations due to a bout of positive selection likely to have occurred circa 20,000 y ago. The dental pleiotropic effects of this allele suggest an even higher occurrence among indigenous people in the Western Hemisphere before European colonization. We hypothesize that selection on EDAR V370A occurred in the Beringian refugium because it increases mammary ductal branching, and thereby may amplify the transfer of critical nutrients in vitamin D-deficient conditions to infants via mothers’ milk. This hypothesized selective context for EDAR V370A was likely intertwined with selection on the fatty acid desaturase (FADS) gene cluster because it is known to modulate lipid profiles transmitted to milk from a vitamin D-rich diet high in omega-3 fatty acids.Publication How strong was the bottleneck associated to the peopling of the Americas? New insights from multilocus sequence data(Sociedade Brasileira de Genética, 2018-01-01) Crawford, Michael H.; Fagundes, Nelson J. R.; Tagliani-Ribeiro, Alice; Rubicz, Rohina; Tarskaia, Larissa; Salzano, Francisco M.; Bonatto, Sandro L.In spite of many genetic studies that contributed for a deep knowledge about the peopling of the Americas, no consensus has emerged about important parameters such as the effective size of the Native Americans founder population. Previous estimates based on genomic datasets may have been biased by the use of admixed individuals from Latino populations, while other recent studies using samples from Native American individuals relied on approximated analytical approaches. In this study we use resequencing data for nine independent regions in a set of Native American and Siberian individuals and a full-likelihood approach based on isolation-with-migration scenarios accounting for recent flow between Asian and Native American populations. Our results suggest that, in agreement with previous studies, the effective size of the Native American population was small, most likely in the order of a few hundred individuals, with point estimates close to 250 individuals, even though credible intervals include a number as large as ~4,000 individuals. Recognizing the size of the genetic bottleneck during the peopling of the Americas is important for determining the extent of genetic markers needed to characterize Native American populations in genome-wide studies and to evaluate the adaptive potential of genetic variants in this population.Publication Genome of the Ancient One (a.k.a. Kennewick Man)(Wayne State University Press, 2015-04-01) Raff, JenniferPublication Paternal Genetic Structure in Contemporary Mennonite Communities from the American Midwest(Wayne State University Press, 2016) Beaty, Kristine G.; Mosher, M. J.; Crawford, Michael H.; Melton, Phillip EdwardOver the last 35 years, researchers from the Laboratory of Biological Anthropology at the University of Kansas have been working with Mennonite communities to better understand evolutionary patterns of fission-fusion in relationship to their genetic history and population structure. In this study, short tandem repeat (STR) markers from the nonrecombining region of the Y chromosome (NRY) provided increased resolution of the molecular population structure for these groups. NRY is known to be informative for determining paternal genetic ancestral patterns in recently derived human populations. Mennonites represent a branch of the Anabaptist movement that began in northern and central Europe in the 16th century and maintain a well-documented migration and genealogical history. Provided this historical information, we investigated the genetic relationship of 15 NRY STR loci within five Mennonite communities from Kansas (Goessel, Lone Tree, Garden View, and Meridian) and Nebraska (Henderson). We sought to determine if patterns of fission/fusion along familial lines persisted with paternal genetic information as evidenced through other classical genetic polymorphisms and molecular markers. NRY haplotype information was obtained for 94 individuals, and genetic variation was analyzed and compared across the five study populations and comparative Anabaptist and European populations. NRY haplogroups were assigned using a Bayesian allele frequency approach with 14 STR loci. A total of 92 NRY haplotypes were detected, with none shared across these communities. The most prevalent NRY haplogroup was R1b, which occurred in 56% of the entire sample. Eight additional NRY haplogroups (E1b1b, G2a, I1, I2, J2a1, L, Q, and R1a) were detected in smaller frequencies. Principal component analysis of NRY data, in contrast to mitochondrial DNA data, displayed no patterns of population subdivision of these congregations into communities. These NRY genetic profiles provide additional information regarding the recent migratory history of Mennonite communities and additional evidence for fission along paternal lines after migration to the United States.Publication Patterns of DNA Methlyation across the Leptin Core Promoter in Four Diverse Asian and North American Populations(Wayne State University Press, 2016) Mosher, M. J.; Melton, Phillip Edward; Schanfield, M. S.; Crawford, Michael H.DNA methylation is the most widely studied of epigenetic mechanisms, with environmental effects recorded through patterned attachments of methyl groups along the DNA that are capable of modifying gene expression without altering the DNA sequencing. The degree to which these patterns of DNA methylation are heritable, the expected range of normality across populations, and the phenotypic relevance of pattern variation remain unclear. Genes regulating metabolic pathways appear to be vulnerable to ongoing nutritional programming over the life course, as dietary nutrients are significant environmental determinants of DNA methylation, supplying both the methyl groups and energy to generate the methylation process. Here we examine methylation patterns along a region of the metabolic gene leptin (LEP). LEP's putative functions include regulation of energy homeostasis, with its signals affecting energy intake and expenditure, adipogenesis and energy storage, lipid and glucose metabolism, bone metabolism, and reproductive endocrine function. A pattern of differential methylation across CpG sites of the LEP core promoter has been previously identified; however, any consistency of pattern or its phenotypic significance is not fully elucidated among populations. Using DNA extracted from unfractionated white blood cells of peripheral blood samples, our pilot study, divided into two parts, examined the significance of variation in DNA methylation patterns along the leptin core promoter in four populations (phase 1) and used biomarkers reflecting leptin's functional process in two of those populations, western Buryat of Siberia and the Mennonite of central Kansas, to investigate the relevance of the ethnic variation identified in the DNA methylation (phase 2). LEP's core promoter region contains both the binding site for C/EBPα (CCAAT/enhancer binding protein alpha), which tempers the final step in adipocyte maturity and capacity to synthesize leptin, and the TATA motif controlling leptin synthesis. Previous studies report that increased methylation in this region is correlated to decreased gene expression, suggesting tissue-specific methylation variation at this region (Melzner et al. 2002). We hypothesized that evidence of nutritional epigenetic programming would be identified through variation in patterns of DNA methylation and that functional relevance of that variation among populations would be identified through biomarkers that reflect leptin's metabolic signals: serum leptin levels, lipoproteins of the lipid transport system, and anthropometric measures. In phase 1, our combined analyses of 313 individuals documented a distinct and consistent overall pattern of differential DNA methylation across seven CpG sites of LEP core promoter in all ethnicities and both sexes. This pattern replicates those identified in previous studies, suggesting a conserved core promoter region across populations. Phase 2 analyses of two of the four populations (n = 239), correlating methylation at the C/EBPα transcription binding site (TBS) with metabolic and anthropometric biomarkers reflecting LEP roles, showed that stature, which reflects bone growth and remodeling, was significantly and inversely correlated with the percentage of DNA methylation at this site in both sexes. We suggest that variation in DNA methylation along the LEP core promoter plays a substantial role in energy signals affecting both adipogenesis and bone metabolism.Publication The Fortieth Anniversary of the Founding of the Laboratory of Biological Anthropology(Wayne State University Press, 2016) Crawford, Michael H.Publication Genetic Affiliation of Pre-Hispanic and Contemporary Mayas through Maternal Linage(Wayne State University Press, 2016) Ochoa-Lugo, Mirna Isabel; Muñoz, María de Lourdes; Pérez-Ramírez, Gerardo; Beaty, Kristine G.; López-Armenta, Mauro; Cervini-Silva, Javiera; Moreno-Galeana, Miguel; Meza, Adrián Martínez; Ramos, Eduardo; Crawford, Michael H.; Romano-Pacheco, ArturoMaya civilization developed in Mesoamerica and encompassed the Yucatan Peninsula, Guatemala, Belize, part of the Mexican states of Tabasco and Chiapas, and the western parts of Honduras and El Salvador. This civilization persisted approximately 3,000 years and was one of the most advanced of its time, possessing the only known full writing system at the time, as well as art, sophisticated architecture, and mathematical and astronomical systems. This civilization reached the apex of its power and influence during the Preclassic period, from 2000 BCE to 250 CE. Genetic variation in the pre-Hispanic Mayas from archaeological sites in the Mexican states of Yucatan, Chiapas, Quintana Roo, and Tabasco and their relationship with the contemporary communities in these regions have not been previously studied. Consequently, the principal aim of this study was to determine mitochondrial DNA (mtDNA) variation in the pre-Hispanic Maya population and to assess the relationship of these individuals with contemporary Mesoamerican Maya and populations from Asia, Beringia, and North, Central, and South America. Our results revealed interactions and gene flow between populations in the different archaeological sites assessed in this study. The mtDNA haplogroup frequency in the pre-Hispanic Maya population (60.53%, 34.21%, and 5.26% for haplogroups A, C, and D, respectively) was similar to that of most Mexican and Guatemalan Maya populations, with haplogroup A exhibiting the highest frequency. Haplogroup B most likely arrived independently and mixed with populations carrying haplogroups A and C based on its absence in the pre-Hispanic Mexican Maya populations and low frequencies in most Mexican and Guatemalan Maya populations, although this also may be due to drift. Maya and Ciboneys sharing haplotype H10 belonged to haplogroup C1 and haplotype H4 of haplogroup D, suggesting shared regional haplotypes. This may indicate a shared genetic ancestry, suggesting more regional interaction between populations in the circum-Caribbean region than previously demonstrated. Haplotype sharing between the pre-Hispanic Maya and the indigenous populations from Asia, the Aleutian Islands, and North, Central, and South America provides evidence for gene flow from the ancestral Amerindian population of the pre-Hispanic Maya to Central and South America.Publication Population History and Mitochondrial Genetic Substructure of the Rama Amerindians from Nicaragua(Wayne State University Press, 2016) Baldi Salas, Norberto Francisco; Crawford, Michael H.The Rama are a coastal population from southern Nicaragua who in large part were able to resist, at least for a time, the cultural changes and social reorganization brought on by colonial and modern influences. Historical information leaves the Rama origins and biological relationships with nearby extinct and extant groups ambiguous. The objective of this study was to examine the internal genetic microdifferentiation based on the first hypervariable region of the mitochondrial DNA (mtDNA) from a sample of approximately 20% of the population, and to expand the few available historical and anthropological data on the Rama by exploring the effects of cultural practices and historical events on genetic structure, providing an integrative perspective on the Rama genetic history. When considering differences in the spatial distribution and genetic diversity of the mtDNA haplotypes together with historical information on the Rama, a noteworthy pattern emerges. (a) Haplotypes are differentially distributed among a central Rama community (Punta Aguila) compared with the other five peripheral communities (analysis of molecular variance: FCT = 0.10, p < 0.001), and their distribution is consistent with the historical relocation of this population after their split from Punta Gorda in the 18th century. (b) Differential genetic signatures found among central and peripheral Rama communities resemble two population histories: one of stability (haplogroup A2) and other of expansion (haplogroup B2), supporting the possibility that these patterns of genetic microdifferentiation between central and peripheral populations resulted from the 18th-century unification in southern Nicaragua of the Rama and a group of Voto migrants from Costa Rica that later split off and moved to the Bay of Bluefields.Publication Views, Barriers, and Suggestions for Colorectal Cancer Screening Among American Indian Women Older Than 50 Years in the Midwest(SAGE Publications, 2013-07-01) Filippi, Melissa K.; James, Aimee S.; Brokenleg, Sarah; Talawyma, Myrietta; Perdue, David G.; Choi, Won S.; Greiner, K. Allen; Daley, Christine MakoskyOBJECTIVE: Although colorectal cancer (CRC) mortality rates in the US population have shown a decline, American Indian (AI) CRC mortality rates appear to be increasing. CRC screening rates of AIs remain low when compared with other ethnic groups. The research team explored women's perceptions toward CRC screening, existing barriers, and suggestions to promote education and screening among AI women in Kansas and Missouri. METHODS: Using a community-based participatory research approach, the authors conducted 7 focus groups with AI women older than 50 years (N = 52) to better understand their perceptions of and attitudes toward CRC screening. RESULTS: Women recognized barriers to screening, such as embarrassment, privacy issues, fear, insurance, and cost. They countered perceived barriers through inventive suggestions for education and awareness via social support systems and intergenerational relationships. DISCUSSION: CRC screening interventions for AI must be culturally tailored.