Vaccine-Induced Immunity in Baboons by Using DNA and Replication-Incompetent Adenovirus Type 5 Vectors Expressing a Human Immunodeficiency Virus Type 1 gag Gene
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Issue Date
2003-07Author
Casimiro, Danilo R.
Tang, Aimin
Chen, Ling
Fu, Tong-Ming
Evans, Robert K.
Davies, Mary-Ellen
Freed, Daniel C.
Hurni, William
Aste-Amezaga, Jose M.
Guan, Liming
Long, Romnie
Huang, Lingyi
Harris, Virginia
Nawrocki, Denise K.
Mach, Henryk
Troutman, Robert D.
Isopi, Lynne
Murthy, Krishna K.
Rice, Karen
Wilson, Keith A.
Volkin, David B.
Emini, Emilio A.
Shiver, John W.
Publisher
American Society for Microbiology
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
The cellular immunogenicity of formulated plasmid DNA and replication-defective human adenovirus serotype 5 (Ad5) vaccine vectors expressing a codon-optimized human immunodeficiency virus type 1 gag gene was examined in baboons. The Ad5 vaccine was capable of inducing consistently strong, long-lived CD8+-biased T-cell responses and in vitro cytotoxic activities. The DNA vaccine-elicited immune responses were weaker than those elicited by the Ad5 vaccine and highly variable; formulation with chemical adjuvants led to moderate increases in the levels of Gag-specific T cells. Increasing the DNA-primed responses with booster doses of either Ad5 or modified vaccinia virus Ankara vaccines suggests a difference in the relative levels of cytotoxic and helper responses. The implications of these results are discussed.
Description
This is the published version. Copyright 2003 American Society for Microbiology.
Collections
- Pharmacy Scholarly Works [293]
Citation
Casimiro, D. R., A. Tang, L. Chen, T.-M. Fu, R. K. Evans, M.-E. Davies, D. C. Freed, W. Hurni, J. M. Aste-Amezaga, L. Guan, R. Long, L. Huang, V. Harris, D. K. Nawrocki, H. Mach, R. D. Troutman, L. A. Isopi, K. K. Murthy, K. Rice, K. A. Wilson, D. B. Volkin, E. A. Emini, and J. W. Shiver. "Vaccine-Induced Immunity in Baboons by Using DNA and Replication-Incompetent Adenovirus Type 5 Vectors Expressing a Human Immunodeficiency Virus Type 1 Gag Gene." Journal of Virology 77.13 (2003): 7663-668. http://dx.doi.org/10.1128/JVI.77.13.7663-7668.2003.
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