TINA interacts with the NIMA kinase in Aspergillus nidulans and negatively regulates astral microtubules during metaphase arrest
View/ Open
Issue Date
2003-08-01Author
Osmani, Aysha H.
Davies, Jonathan
Oakley, C. Elizabeth
Oakley, Berl R.
Osmani, Stephen A.
Publisher
American Society for Cell Biology
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
The tinA gene of Aspergillus nidulans encodes a protein that interacts with the NIMA mitotic protein kinase in a cell cycle-specific manner. Highly similar proteins are encoded in Neurospora crassa and Aspergillus fumigatus. TINA and NIMA preferentially interact in interphase and larger forms of TINA are generated during mitosis. Localization studies indicate that TINA is specifically localized to the spindle pole bodies only during mitosis in a microtubule-dependent manner. Deletion of tinA alone is not lethal but displays synthetic lethality in combination with the anaphase-promoting complex/cyclosome mutation bimE7. At the bimE7 metaphase arrest point, lack of TINA enhanced the nucleation of bundles of cytoplasmic microtubules from the spindle pole bodies. These microtubules interacted to form spindles joined in series via astral microtubules as revealed by live cell imaging. Because TINA is modified and localizes to the spindle pole bodies at mitosis, and lack of TINA causes enhanced production of cytoplasmic microtubules at metaphase arrest, we suggest TINA is involved in negative regulation of the astral microtubule organizing capacity of the spindle pole bodies during metaphase.
Description
This is the publisher's version, also available electronically from "www.molbiolcell.org".
Collections
Citation
Osmani, A.H., Davies, J., Oakley, C., Oakley, B. & Osmani, S.A. (2003). TINA interacts with the NIMA kinase in Aspergillus nidulans and negatively regulates astral microtubules during metaphase arrest. Molecular Biology of the Cell, 14(8), 3169-3179. http://www.dx.doi.org/10.1091/mbc.E02-11-0715
Items in KU ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
We want to hear from you! Please share your stories about how Open Access to this item benefits YOU.