Polyribosome targeting to microtubules: enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos
Issue Date
1994-11-15Author
Hamill, Danielle R.
Davis, Jill
Drawbridge, Julie
Suprenant, Kathy A.
Publisher
Rockefeller University Press
Type
Article
Article Version
Scholarly/refereed, publisher version
Metadata
Show full item recordAbstract
A subset of mRNAs, polyribosomes, and poly(A)-binding proteins copurify with microtubules from sea urchin embryos. Several lines of evidence indicate that the interaction of microtubules with ribosomes is specific: a distinct stalk-like structure appears to mediate their association; ribosomes bind to microtubules with a constant stoichiometry through several purification cycles; and the presence of ribosomes in these preparations depends on the presence of intact microtubules. Five specific mRNAs are enriched with the microtubule-bound ribosomes, indicating that translation of specific proteins may occur on the microtubule scaffolding in vivo.
Description
This is the publisher's version, also available electronically from http://jcb.rupress.org/content/127/4/973.
ISSN
0021-9525Collections
Citation
Hamill, D.; Davis, J.; Drawbridge, J.; Suprenant, Kathy A. (1994). "Polyribosome targeting to microtubules: enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos." Journal of Cell Biology, 127(3-4):973-984. http://www.dx.doi.org/10.1083/jcb.127.4.973.
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